As others have suggested, it looks very much like a substitution at the
fluorine position. I might add that high resolution electrospray mass spec
should be very useful in this case, because I suspect that this adduct will
survive sample preparation, and it would provide strong and orthogonal
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] A question about Cys and fluoro benzene ring
Dear Cheng,
it is possible that the sulfhydryl of the cysteine might have reacted with the
methyl fluoro benzyl group via nucleophilic aromatic substitution. Fluorine is
not the best leaving group among
Dear Cheng,
it is possible that the sulfhydryl of the cysteine might have reacted with the
methyl fluoro benzyl group via nucleophilic aromatic substitution. Fluorine is
not the best leaving group among the halides but it would certainly allow this
especially if the ligand-protein incubation
