[ccp4bb] AW: [ccp4bb] ANISOU in pdb and density improvement

by the UCLA anisotropy server. Using the scaled mtz should solve your problem. Best, Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Appu kumar Gesendet: Donnerstag, 2. Juli 2015 05:17 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] ANISOU in pdb and density improvement

Re: [ccp4bb] ANISOU in pdb and density improvement

Hello All, Sorry for giving incomplete information. The protein is 75KDa membrane protein and it exists as tetramer in ASU. Structure is solved by MR. Overall completeness of data is 98% wiith multiplicity of 4.8. Density looks great after refinement having ANISOU record in PDB. Any suggestions

[ccp4bb] ANISOU in pdb and density improvement

Dear CCP4 users, I am refining a structure at 4A resolution. Crystal diffracted anisotropically and after refinement in both PHENIX and REFMAC, electron density in one of the domain of protein is represented by discontinuity and poor maps. Therefore i did the aniosotropy correction using

Re: [ccp4bb] ANISOU in pdb and density improvement

4 Angstrom resolution is pretty low and there has to be other info associated with that to get more help from here. How big is your protein? How are you solving the phases? How complete is your data at that resolution? What kind of multiplicity are you getting? I think you have other issues that