[ccp4bb] AW: [ccp4bb] Negative electron density in the Fo-Fc map at the binding site

2014-07-22 Thread Herman . Schreuder
Dear Armando, Is 1.9Å really the diffraction limit of your crystals, or do they diffract further and is 1.9Å just a convenient resolution cutoff? In the latter case you might be looking at truncation effects. Best, Herman -Ursprüngliche Nachricht- Von: CCP4 bulletin board

Re: [ccp4bb] AW: [ccp4bb] Negative electron density in the Fo-Fc map at the binding site

2014-07-22 Thread Eleanor Dodson
Well yes, The bulk solvent model can distort the density, especially if the ligand is large. But it usually isnt too serious - try doing SIMPLE scaling and see if that has any effect on the appearance of the density Eleanor On 22 July 2014 10:16, herman.schreu...@sanofi.com wrote: Dear