I also have to come in defense of the nanodrop here.
I have measured up to A280 = 98 and the curve is always reasonably
smooth, spikes normally mean that bubbles have formed. And proper
cleaning seems to be rubbing with a kimwipe three or four times
after each drop. If the last user does
Cuesta-Seijo
Sent: Monday, December 08, 2008 12:54 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] AW: [ccp4bb] suggestions for UV spectrometer
I also have to come in defense of the nanodrop here.
I have measured up to A280 = 98 and the curve is always reasonably
smooth, spikes normally mean
_
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Juergen Bosch
Sent: Saturday, December 06, 2008 12:29 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] AW: [ccp4bb] suggestions for UV spectrometer
Hi,
really strange, I always dilute my protein when taking an absorption
Agree!
I think for crystallographic use the nanodrop is perfectly okay to see if
the protein is 5mg/ml or 30mg/ml. But in fact I also do not trust our
instrument if it comes to more important issues like preparing solutions for
titrations or assays. And due to the small pathlength I do not trust
Which brings us back to the Hellma TrayCell solution where you can,
from the same spectrometer, have both the cuvette option and the
quickness of the NanoDrop/NanoVue system.
Anyone that can comment on the performance of the TrayCell from Hellma?
Cheers,
Martin
On Dec 5, 2008, at 9:06 AM,
