Hi Mohammad,
I'm not sure anyone here can give you absolute answers, because we don't
know enough about your system, but there's 3 things you might want to
explore:
1. Contaminating proteases, even at tiny amounts, could lead to appreciable
cleavage of your protein over the length of a
@JISCMAIL.AC.UK
Subject: [ccp4bb] Auto-proteolysis
Dear all,
I am working on a His-tag recombinant protein with two domains, which I purify using affinity chromatography. When I set up crystallization of the same, it gave me crystals in two different conditions- one was the complete protein
Extraordinary conclusions require extraordinary proof, yes?
If you are completely convinced (!) that auto-proteolysis is possible
(meaning that the enzyme you're studying is at least a proteinase in
potentia, and also you have utterly ruled out contamination by host
proteases) then the next step
Dear all,
I am working on a His-tag recombinant protein with two domains, which I
purify using affinity chromatography. When I set up crystallization of the
same, it gave me crystals in two different conditions- one was the complete
protein. The other just had the Domain2.
Even on SDS-PAGE, I
