Re: [ccp4bb] B-factors, H/D exchange and protein flexibility

2007-11-13 Thread Jayashankar
Dear Brad, the flexibility, that is how far a protein is stabilized with cold and heat denaturation of a protein, this inturn depends on 1.solvation enthalpy of nonpolar moieties(need more energy,so there needs to be some randomness) 2.shift in temparature due to the enthalpy associated with tra

Re: [ccp4bb] B-factors, H/D exchange and protein flexibility

2007-11-13 Thread Ethan Merritt
On Tuesday 13 November 2007 06:41, Brad Bennett wrote: > > I would be interested then to know how the community feels about the > correlation of B-factors to protein flexibility. It is generally accepted > that these are linked but are there any new papers that address this? This is the basis of

[ccp4bb] B-factors, H/D exchange and protein flexibility

2007-11-13 Thread Brad Bennett
Hello all: There are studies, some much older and some relatively new, by Kossiakoff, Karplus and others showing that B-factors of amide nitrogen atoms are not correlated to their hydrogen/deuterium exchange behavior. Along similar lines, I would be interested then to know how the community feels