Hi Folks,
Are you any favorite brands out there for crack-resistant 50mL
centrifugation tubes. It seems we are having recurring episodes of Falcon
and Corning tubes cracking even at 9,000 rpm, which is the maximum speed
possible with our rotor. I have used Falcon tubes for years in the past
To how many g does your 9000 rpm translate ? Perhaps that's the problem ?
10 minutes @ 5000xg for pelleting cells is more than enough in my opinion.
Jürgen
On Jan 31, 2012, at 11:59 AM, Raji Edayathumangalam wrote:
Hi Folks,
Are you any favorite brands out there for crack-resistant 50mL
9000 rpm translates to 13,000 g on this centrifuge/rotor.
I am not referring to pelleting bacterial cells. My question is about
centrifuging bacterial lysates and some recommendations for sturdy tubes.
Thanks.
Raji
On Tue, Jan 31, 2012 at 12:02 PM, Bosch, Juergen jubo...@jhsph.edu wrote:
To
Sorry I misunderstood that, I thought you wanted to pellet cells.
Well in that case my reply would have been get some 50ml Nalgene tubes (I think
they are good to 5xg) for an SS-34 rotor and spin your lysate at the
maximum g force you can get for 30 minutes to get a good pellet. This will
Raji, we use the 50 ml Cornings up to 25K RCF in our F13S-14x50cy rotor without
leakage so it could be a batch specific problem with your Corning tubes. Try to
talk to Piramoon or one their resellers (Thermo?) in your country if they have
some batches of 50 ml tubes in stock that they can
In our hands the polyethylene tubes work even at 11krpm or over 30kg
forces. But NOT polycarbonate ones.
On Jan 31, 2012 10:59 AM, Raji Edayathumangalam r...@brandeis.edu wrote:
Hi Folks,
Are you any favorite brands out there for crack-resistant 50mL
centrifugation tubes. It seems we are
