Dear crystallographers
Trying to crystallize a membrane protein complex of 100 kDa with a soluble
protein of 20 kDa which is interact with the membrane protein. So far, no
co-crystals in 200 conditions. Some conditions gave crystals but mass spec of
crystals show only either one protein
I would advise against using anti-His FaBs for that particular purpose.
Even if you get them to bind to your complex, the inherent flexibility of
the terminal regions were His-tags are placed might ruin the final result,
and they're not cheap to begin with.
You can further characterize your
Try seeding the complex with the crystals that you have. If you have
crystals of both proteins, crush them and mix them together. Suspend
the seeds in whichever reservoir solution has the least salt in it*
(or suspend in 50% PEG**) . Use random Microseed Matrix Screening
(rMMS) i.e.
of our computer systems and checking Compliance with our Code of
Conduct and Policies.
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Theresa
Hsu
Sent: 28 June 2012 13:26
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystallization with antibody
