@JISCMAIL.AC.UK] On Behalf Of
dusky dew
Sent: Saturday, May 03, 2014 1:29 AM
To: CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Crystals Disappearing Overnight
Thank you all for getting back!
I will set up the drops using microbatch method.
Regarding the temp, I set them
@JISCMAIL.AC.UK] On Behalf Of
dusky dew
Sent: Saturday, May 03, 2014 1:29 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Crystals Disappearing Overnight
Thank you all for getting back!
I will set up the drops using microbatch method.
Regarding the temp, I set them up in lab and put them
:22 -0700
Subject: Re: [ccp4bb] Crystals Disappearing Overnight
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I tried microbatch and the crystals are not stable. They dissolve
: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
dusky dew
Sent: Friday, May 02, 2014 4:39 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystals Disappearing Overnight
Dear All,
I am trying to crystallize a protein with Adenosine. My protein is in 20
mM Tris, 300 mM
Dear All,
I am trying to crystallize a protein with Adenosine. My protein is in 20
mM Tris, 300 mM NaCl and the crystals appear in a condition with 5 percent
PEG8K, 0.1 M Sodium Cacodylate. The protein is incubated with adenosine
for 1/2 hr before setting the drop. The crystals appear right
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Dear Maria,
this is similar to the condition I used for my PhD thesis. I suppose
the following happens:
when you mix the drop the protein crystallises by inverse salting-in
because you dilute the high-salt concentration. This happens
instantaneously
Dear Tim,
you are right that 150mM NaCl is 'stronger' than 2.5% PEG. If you
calculate the relative humidity difference between 150 and 300mM Nacl
you get 99.5 vs 98.9% whereas the difference between 5 and 2.5% P8K is
99.96 vs 99.99% ie virtually nothing. You can calculate these values
here:
Hi Maria -
I think you already have a likely answer, but here's another one which I
have observed many times:
Temperature differences. If your crystallization plate is moved from
one temperature to another (lab vs. crystal incubator), or even if it's
left on the bench but the lab
