.
Thanks again to all you,
Christine
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Enrico Stura
Sent: Monday, November 28, 2011 1:45 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Disappearing crystals
When advice on crystallization is needed
@JISCMAIL.AC.UK] On Behalf Of
Enrico Stura
Sent: Monday, November 28, 2011 1:45 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Disappearing crystals
When advice on crystallization is needed, it is important to give details
of
the protein concentration, the buffer the protein is in as well
On 11/28/11 12:04, Harman, Christine wrote:
Hi All,
I have just noticed a very strange thing and need some help in
understanding it. I recently found two crystals in a condition from a
screen (0.05M Calcium chloride dihydrate, 0.1M M Bis-Tris pH6.5 and
30% PEG MME 550). The small crystals
On 11/28/11 12:04 PM, Harman, Christine wrote:
Hi All,
I have just noticed a very strange thing and need some help in
understanding it. I recently found two crystals in a condition from a
screen (0.05M Calcium chloride dihydrate, 0.1M M Bis-Tris pH6.5 and
30% PEG MME 550). The small
When advice on crystallization is needed, it is important to give details
of
the protein concentration, the buffer the protein is in as well as the
method
used to grow the crystals.
Problem: The crystallization conditions are essentially low salt: 100mM
buffer
and only 50mM CaCl2. So the
,
Christine
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Enrico
Stura
Sent: Monday, November 28, 2011 1:45 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Disappearing crystals
When advice on crystallization is needed, it is important to give
]
Sent: Monday, November 28, 2011 12:43 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Disappearing crystals
Hi,
Thank you so much for your replies. A lot of you have mentioned fluctuations
in temp as the major contributor. And a few of you have asked for more details
of my protein/buffer
...@fda.hhs.gov]
Sent: Monday, November 28, 2011 12:43 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Disappearing crystals
Hi,
Thank you so much for your replies. A lot of you have mentioned
fluctuations in temp as the major contributor. And a few of you have asked
for more details of my
