Dear Gloria,
I think that the most economical biotinylation manner is in vivo, by
co-expression of a tagged protein with BirA. One can PCR amplify the BirA cDNA
from E. coli and clone it into plasmids for any expression system and
sub-cellular localization needed. Ready to use BirA expression
Linda
From: CCP4 bulletin board on behalf of Gloria Borgstahl
Sent: Monday, October 21, 2019 12:22 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] E. coli BirA biotin ligase expression/purification
ATTENTION: This email originated from a sender outside of MCW. Use
Dear friends in crystallography,
I know this seems unrelated, but it really isn't ... please forgive me.
We are trying to use the Avitag/BirA system to specifically biotinylate
target proteins in an economical manner. Are any of you purifying the BirA
enzyme in your lab for biotinylation and if
