Subject: Re: [ccp4bb] Enhancing Crystal Quality
Hi,
Thanks for the kindly answers from everyone. I actually haven't tried
different cryoprotectants. I might will give a try next time. I usually
only use mother liquor+30% PEG400. It is noticeable that it has some
patterns (cracks
@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Enhancing Crystal Quality
Hi,
Thanks for the kindly answers from everyone. I actually haven't tried
different cryoprotectants. I might will give a try next time. I usually
only use mother liquor+30% PEG400. It is noticeable that it has some
: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Yi-Liang Liu
Sent: Thursday, August 02, 2012 4:15 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Enhancing Crystal Quality
Hi,
Thanks for the kindly answers from everyone. I actually haven't tried
different cryoprotectants. I
drying out.
Good luck!
Herman
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Yi-Liang Liu
Sent: Thursday, August 02, 2012 4:15 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Enhancing Crystal Quality
Hi,
Thanks for the kindly
to prevent the crystal from drying out.
Good luck!
Herman
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Yi-Liang Liu
Sent: Thursday, August 02, 2012 4:15 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Enhancing Crystal Quality
Hi CCP4BBers,
I have a protein crystallized with 0.2mM Mg acetate and PEG 8000 or 0.1mM
cacodylate, pH 6.5, 0.2 Mg acetate, PEG 3350. Both of the conditions gave
triangle pyramid like crystals. I brought the crystals to synchrotron using 30%
PEG 400 as cryoprotactant, the resolution was only
Have you tried different cryoprotectants? Can make a huge difference. Also,
have you shot an xtal at room temp - to see what the intrinsic diffraction
limit is? Additive screens? If all else fails you may well need to explore a
different expression construct.
Tony.
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On 1
Hi,
Thanks for the kindly answers from everyone. I actually haven't tried different
cryoprotectants. I might will give a try next time. I usually only use mother
liquor+30% PEG400. It is noticeable that it has some patterns (cracks (?)) on
the crystal. However, it didn't form icy rings or etc.