Re: [ccp4bb] Enhancing Crystal Quality

2012-08-13 Thread Sangeetha Vedula
Subject: Re: [ccp4bb] Enhancing Crystal Quality Hi, Thanks for the kindly answers from everyone. I actually haven't tried different cryoprotectants. I might will give a try next time. I usually only use mother liquor+30% PEG400. It is noticeable that it has some patterns (cracks

Re: [ccp4bb] Enhancing Crystal Quality

2012-08-12 Thread Bosch, Juergen
@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Enhancing Crystal Quality Hi, Thanks for the kindly answers from everyone. I actually haven't tried different cryoprotectants. I might will give a try next time. I usually only use mother liquor+30% PEG400. It is noticeable that it has some

Re: [ccp4bb] Enhancing Crystal Quality

2012-08-02 Thread Herman . Schreuder
: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Yi-Liang Liu Sent: Thursday, August 02, 2012 4:15 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Enhancing Crystal Quality Hi, Thanks for the kindly answers from everyone. I actually haven't tried different cryoprotectants. I

Re: [ccp4bb] Enhancing Crystal Quality

2012-08-02 Thread Yi-Liang Liu
drying out. Good luck! Herman -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Yi-Liang Liu Sent: Thursday, August 02, 2012 4:15 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Enhancing Crystal Quality Hi, Thanks for the kindly

Re: [ccp4bb] Enhancing Crystal Quality

2012-08-02 Thread Roger Rowlett
to prevent the crystal from drying out. Good luck! Herman -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Yi-Liang Liu Sent: Thursday, August 02, 2012 4:15 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Enhancing Crystal Quality

[ccp4bb] Enhancing Crystal Quality

2012-08-01 Thread Yi-Liang Liu
Hi CCP4BBers, I have a protein crystallized with 0.2mM Mg acetate and PEG 8000 or 0.1mM cacodylate, pH 6.5, 0.2 Mg acetate, PEG 3350. Both of the conditions gave triangle pyramid like crystals. I brought the crystals to synchrotron using 30% PEG 400 as cryoprotactant, the resolution was only

Re: [ccp4bb] Enhancing Crystal Quality

2012-08-01 Thread Antony Oliver
Have you tried different cryoprotectants? Can make a huge difference. Also, have you shot an xtal at room temp - to see what the intrinsic diffraction limit is? Additive screens? If all else fails you may well need to explore a different expression construct. Tony. Sent from my iPhone On 1

Re: [ccp4bb] Enhancing Crystal Quality

2012-08-01 Thread Yi-Liang Liu
Hi, Thanks for the kindly answers from everyone. I actually haven't tried different cryoprotectants. I might will give a try next time. I usually only use mother liquor+30% PEG400. It is noticeable that it has some patterns (cracks (?)) on the crystal. However, it didn't form icy rings or etc.