[ccp4bb] Expression of a Selenomethionine Variant in E. coli

2011-12-12 Thread Yibin Lin
Hi List, I want to preprare sel-met labeling protein. Could someone can tell me if it is necessary to add DTT or BME to medium, which contains sel-met, when growing cells? Thanks in advance, Yibin Lin

Re: [ccp4bb] Expression of a Selenomethionine Variant in E. coli

2011-12-12 Thread Antony Oliver
Generally no, only in the subsequent protein purification steps. Tony. Sent from my iPhone On 12 Dec 2011, at 21:57, Yibin Lin yyb...@gmail.com wrote: Hi List, I want to preprare sel-met labeling protein. Could someone can tell me if it is necessary to add DTT or BME to medium, which