[ccp4bb] Fab purification and crystallization

2010-09-10 Thread Patrick Shaw Stewart
...@jiscmail.ac.uk] On Behalf Of xaravich ivan Sent: 10 September 2010 04:00 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Fab purification and crystallization Hi CCP4bb, I have two questions regarding Fab purification and Fab-antigen complex crystallization and would really appreciate any input

Re: [ccp4bb] Fab purification and crystallization

2010-09-10 Thread xaravich ivan
-9090US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 *From:* CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] *On Behalf Of *xaravich ivan *Sent:* 10 September 2010 04:00 *To:* CCP4BB@JISCMAIL.AC.UK *Subject:* [ccp4bb] Fab purification and crystallization

Re: [ccp4bb] Fab purification and crystallization

2010-09-10 Thread Zhijie Li
in the original purification buffer supplemented with EDTA and cysteine? Zhijie From: xaravich ivan Sent: Thursday, September 09, 2010 10:59 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Fab purification and crystallization Hi CCP4bb, I have two questions regarding Fab purification

Re: [ccp4bb] Fab purification and crystallization

2010-09-09 Thread Jürgen Bosch
I assume you separate your complex over a size exclusion before setting up trays ? If not try that and see if you get better crystals. Jürgen - Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry Molecular Biology Johns Hopkins Malaria Research Institute 615

Re: [ccp4bb] Fab purification and crystallization

2010-09-09 Thread Sivaraman Padavattan
Dear Ivan, We faced the same problem for our Hya/Fab complex (clustered needles). By change the pH and doing microseeding, we were able to improve the crystals quality. For more information see our paper Padavattan, S., Schirmer, T., Schmidt, M., Akdis, C., Valenta, R., Mittermann, I.,