...@jiscmail.ac.uk] On Behalf Of
xaravich ivan
Sent: 10 September 2010 04:00
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Fab purification and crystallization
Hi CCP4bb,
I have two questions regarding Fab purification and Fab-antigen complex
crystallization and would really appreciate any input
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*From:* CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] *On Behalf Of
*xaravich
ivan
*Sent:* 10 September 2010 04:00
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* [ccp4bb] Fab purification and crystallization
in the original purification buffer supplemented with EDTA and
cysteine?
Zhijie
From: xaravich ivan
Sent: Thursday, September 09, 2010 10:59 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Fab purification and crystallization
Hi CCP4bb,
I have two questions regarding Fab purification
I assume you separate your complex over a size exclusion before setting up
trays ?
If not try that and see if you get better crystals.
Jürgen
-
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry Molecular Biology
Johns Hopkins Malaria Research Institute
615
Dear Ivan,
We faced the same problem for our Hya/Fab complex (clustered needles). By
change the pH and doing microseeding, we were able to improve the crystals
quality. For more information see our paper
Padavattan, S., Schirmer, T., Schmidt, M., Akdis, C., Valenta, R.,
Mittermann, I.,