[ccp4bb] Florets- How to improve it

2009-05-18 Thread james09 pruza
Deal all, Sorry for the non-ccp4 query. I am new to this field and need some suggestions regarding the improvement in the crystallization qual I have crystallized a protein with 65% MPD in 0.1M Acetate buffer pH 3.6 at 4 degree. The florets appear after 2 days and covers the whole drop. What

Re: [ccp4bb] Florets- How to improve it

2009-05-18 Thread Enrico Stura
Re: 65% MPD in 0.1M Acetate buffer pH 3.6 These conditions can crystallize salt impurities present in the protein buffer. The first step is to ensure that the crystals are protein. If they are protein the next step is to reduce the MPD concentration and seed the drops if no crystals appear.