Greetings (or in "correct" Swiss German "Grüezi
wohl", with Umlaut=vowel mutation) to all CCP4BB
subscribers,
being a CCP4BB reader (and sometimes writer)
since something like 15 years, I realized today
that the comment of Gerard was the first one I
read since then containing a side-swipe regarding
the nationality or the national character of
authors.
I think, within a multinational scientific
community with a very long lasting tradition of
internationalism such comments should be beyond
this forum.
On the other hand Gerard's comment contains a
somewhat humorous (Swiss, Swedish, German, Dutch,
... kind of?) aspect, since it refers partly to
Switzerland, a country where many foreigners live
and work, including Germans like Dirk. I would
guess a situation similar like in Sweden or The
Netherlands, isn't it?
Sorry being German too I could not resist making such a moralizing comment.
Coming back to the original question from Shivesh
Kumar, I believe Dirk is right. Shivas' question
was not very specific. He probably simply wanted
to know how to process MAD data correctly e.g.
regarding the anomalous signal, which resulted
sometimes in sign problems when using CCP4
programs (at least with previous versions), if I
remember it correctly. Such questions came up
frequently in the past and were not answered in a
nasty/sarcastic way.
--cd
Dr. Klaus Piontek
Albert-Ludwigs-University Freiburg -ALU-FR-
(ALU-FR is an equal opportunity employer
dedicated to the goal of building a culturally
diverse and pluralistic community with a
multicultural environment)
Institute of Organic Chemistry and Biochemistry, Room 401 H
Albertstrasse 21
D-79104 Freiburg
Germany
Phone: ++49-761-203-6036
Fax: ++49-761-203-8714
Email: [EMAIL PROTECTED]
Web: http://www.chemie.uni-freiburg.de/orgbio/w3platt/
X-Real-To: <[EMAIL PROTECTED]>
X-RAL-MFrom: <[EMAIL PROTECTED]>
X-RAL-Connect: <elvira.its.uu.se [130.238.164.5]>
X-CCLRC-SPAM-report: 0 :
Date: Thu, 1 Mar 2007 10:31:06 +0100
Reply-To: Gerard DVD Kleywegt <[EMAIL PROTECTED]>
Sender: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK>
From: Gerard DVD Kleywegt <[EMAIL PROTECTED]>
Subject: [ccp4bb] Swiss humour - no laughing
matter? (Re: [ccp4bb] process SeMet labelled
data)
Comments: To: Dirk Kostrewa <[EMAIL PROTECTED]>
To: CCP4BB@JISCMAIL.AC.UK
List-Help: <http://www.jiscmail.ac.uk/cgi-bin/webadmin?LIST=CCP4BB>,
<mailto:[EMAIL PROTECTED] CCP4BB>
List-Unsubscribe: <mailto:[EMAIL PROTECTED]>
List-Subscribe: <mailto:[EMAIL PROTECTED]>
List-Owner: <mailto:[EMAIL PROTECTED]>
List-Archive: <http://www.jiscmail.ac.uk/cgi-bin/webadmin?LIST=CCP4BB>
gruzi dirk,
come on - lighten up a little! your reply wasn't
funny or helpful either - just moralising. (by
the way - 't is a strange fact that whenever i
get any negative reactions to my own postings
they stem *exclusively* from any or all of these
three countries: the us, germany and
switzerland. positive reactions, on the other
hand, are typically from (again) the us and the
uk. i think there might be a phd thesis in there
for some cultural anthropologist)
to address your point - we are here to try and
answer queries, not to teach people
crystallography from the ground up. that's what
supervisors are for. and my advice to students
without (access to) crystallographically trained
supervisors would be to apply for a good course
(e.g., the one in cold spring harbor every
autumn), to move elsewhere or to not do
crystallography (so as to avoid mono-, di-,
tri-, tetra- or pentaretractions, which tend to
be a bit of a blot on anyone's cv)
i think this is the point that the people who
replied "carefully" etc. were making, albeit
more succinctly and humourously (albeit a trifle
sarcastically, perhaps). moreover, these replies
came from people who have contributed seriously
to ccp4bb on numerous occasions!
look, we're all busy, and time-waster postings
are annoying. such postings, and moralisations
about "good style", can eventually tee off the
experts who take the time to share their
knowledge and expertise. if joe bloggs, who
never posts anything, is annoyed by one of my
posts and unsubscribes, that's his loss. if,
however, people like tassos unsubscribe it's
*our* loss!
so, dear subscribers: before posting a question
- ask your supervisor and/or your colleagues. if
they can't help you, search the web with google
(in many cases your question will have been
asked, and answered, before). if you're still
not happy, then by all means post a message to
the appropriate bulletin board
darn! look what you've made me do! now i'm
moralising myself! guess i'd better move to
switzerland then ;-)
--dvd
On Thu, 1 Mar 2007, Dirk Kostrewa wrote:
Hi Mark,
although Shivesh's question was not very
specific, and he should have clearly given some
more informations about what he would like to
know, he is probably a beginner in
crystallography and simply asked for help on
this board. Not everyone has always time or is
always in the mood to answer such questions. In
my opinion, it's then better not to respond at
all than to give an answer like yours that is
neither helpful nor funny! We all should try to
keep a good style here.
Dirk.
Mark J. van Raaij wrote:
why don't you just send all your images to the
ccp4bb, then we'll process them, solve the
structure and publish it for you.
And we might put you in the acknowledgements, if you are lucky.
Mark
On 28 Feb 2007, at 16:35, Jonathan Grimes wrote:
Anastassis Perrakis wrote:
On Feb 28, 2007, at 14:37, shivesh kumar wrote:
Dear all,
I have a data set at 2.2A, of the
selenomethionene labelled protein.How
should I process the data.
Carefully !
Thanx for the help.
Shivesh
Tassos
i am sure what tassos really meant was "Very Carefully !"
jon
--
Dr. Jonathan M. Grimes, Royal Society
Research Fellow University Research
Lecturer
Division of Structural Biology
Wellcome Trust Centre for Human Genetics
University of Oxford
Roosevelt Drive,
Oxford OX3 7BN, UK
Email: [EMAIL PROTECTED], Web:
www.strubi.ox.ac.uk Tel: (+44) - 1865 -
287561, FAX: (+44) - 1865 - 287547
Mark J. van Raaij
Dpto de Bioquímica, Facultad de Farmacia
and
Unidad de Rayos X, Edificio CACTUS
Universidad de Santiago
15782 Santiago de Compostela
Spain
http://web.usc.es/~vanraaij/
--
****************************************
Dirk Kostrewa
Paul Scherrer Institut
Biomolecular Research, OFLC/110
CH-5232 Villigen PSI, Switzerland
Phone: +41-56-310-4722
Fax: +41-56-310-5288
E-mail: [EMAIL PROTECTED]
http://sb.web.psi.ch
****************************************
with best regards,
--gerard
******************************************************************
Gerard J. Kleywegt
[Research Fellow of the Royal Swedish Academy of Sciences]
Dept. of Cell & Molecular Biology University of Uppsala
Biomedical Centre Box 596
SE-751 24 Uppsala SWEDEN
http://xray.bmc.uu.se/gerard/ mailto:[EMAIL PROTECTED]
******************************************************************
The opinions in this message are fictional. Any similarity
to actual opinions, living or dead, is purely coincidental.
******************************************************************
--
Dr. Klaus Piontek
Albert-Ludwigs-University Freiburg
Institute of Organic Chemistry and Biochemistry, Room 401 H
Albertstrasse 21
D-79104 Freiburg
Germany
Phone: ++49-761-203-6036
Fax: ++49-761-203-8714
Email: [EMAIL PROTECTED]
Web: http://www.chemie.uni-freiburg.de/orgbio/w3platt/
