Alternatively, you can try soaking with Ln complexes, such as
Tantalum-Br clusters (http://www.mitegen.com/mic_catalog.php?c=jenPhasingTantalum)
Ln chelated in EDTA-like molecules. It does not bind covalently.
Better used for soaking at high concentration
Dear Isa,
Do you have any cysteines? I assume so given you mention class B metals... If
so, and you have a ready supply of relatively isomorphous crystals, I would
consider giving native SAD a go, collecting datasets from 5-10 (or however many
you need) datasets at a reasonably low energy (say
Hi Isa,
don't discard SeMet too rapidly if there are few Mets, modern beamlines,
high-redundancy data collection techniques, and processing and phasing programs
can extract and use small anomalous signal to get structures even if there are
less SeMets than generally accepted by the rule of
Also you can treat your SeMET as heavy atom derivative with your native dataset.
J?rgen
..
J?rgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe
Hi All,
We recently obtained a native data set to 2.8A. With no molecular replacement
available we are now moving to heavy atom (not enough methionine coverage for
seleno-met). Unfortunately crystals grow is in 1.4 Na/K H phosphate pH 8 and
we do not have much room for improvement.
Any
