There are a good many coiled coil models available. I suggest you search
the pdb for more models yourself, or let a program like BALBES do it for
you and do the MR searches as well.
IF you get a faint hit (usually marked by both R and rfree dropping a
few % on refinement) then Arp-warp or
Two cents are added here.
First, try P2 as somethimes systematic absence along b axis is misleading
due to weak diffraction or pseduo translation.
Second, try P1.
Good luck,
Donghui
On Tue, Jan 26, 2010 at 1:50 AM, Michele Lunelli efu...@yahoo.it wrote:
Dear all,
I am trying to solve a
I would guess that coiled-coil structures might be difficult to solve by MR
because of multiple false solutions in a repetitive structure.
There's a lot to be said for experimental phases
Phil
On 25 Jan 2010, at 17:50, Michele Lunelli wrote:
Dear all,
I am trying to solve a structure at
It may be a good exercise to look at the possibility of weak reflections
in the
original images. Such reflections are not picked up by the automatic
methods and
could be a possible source for your problems.
Use manual spots picking in mosflm or an equivalent program. This will
ensure that
Yes, that's true: coiled-coils are a nightmare, especially for molecular
replacement! Apart from potential twinning problems, the internal
symmetry and often very tight packing makes it extremely awful for MR
replacement trials. I would recommend to create at least seven models
for molecular
Dear all,
I am trying to solve a structure at 2.05 A resolution by molecular replacement.
The space group
seems to be P21, with unit cell dimension 52.63, 29.43, 104.970 and beta =
95.60.
Only one copy of the protein should be present in the asymmetric unit, with 58%
of solvent content.
The
