Matthew,
I have not had any problem with our Cryojet XL (90 - 300 Kelvin), it is
very stable at sub-zero temperatures, I checked it over a longer period
using a highly sensitive electronic device. Also, you can buy the
Cryojet HT that can be operated from 90 – 490 Kelvin!
The heaters will be
We used to use FTS-cooler for stable
temperatures in Laue-experiments on
protein crystals in capillaries.
Check out BioCARS at the APS for this device.
http://cars9.uchicago.edu/biocars/pages/timeresolved.html
It has a big opening for constant air flow
and temperature along the capillary and
it
Department of Biochemistry
Henry Wellcome Building
University of Leicester
LE1 9HN
0116 229 7097
From: CCP4 bulletin board on behalf of mesters
Sent: Wed 11/07/2007 08:46
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Help with reducing crystal mosaicity
snipped
-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Mary
Fitzgerald
Sent: 09 July 2007 23:05
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Help with reducing crystal mosaicity
Help please!
I'm looking for some new ideas. I have crystals that come out of a
sitting drop
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] Behalf Of
Patrick Shaw Stewart
Sent: 11 July 2007 12:10
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Help with reducing crystal mosaicity
Just a thought, Mary - going back to your original question about MPD. I
, 2007 7:17 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Help with reducing crystal mosaicity
Dear Mary,
the problem encountered with cryoprotectans is the change in the solution
surrounding your crystal as they may not be present in your crystallisation
conditions or you need to increase
mentioned before).
- J. -
-Original Message-
From: Mary Fitzgerald [EMAIL PROTECTED]
Date: Mon, 9 Jul 2007 18:05:10
To:CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Help with reducing crystal mosaicity
Help please!
I'm looking for some new ideas. I have crystals that come out
Jeroen brings up a good point. Back in the old days, around 5 B. C.
(Before Cryo), we would use a chilled air generator to blow a stream
of cold air along the capillary axis to keep the crystals just above
their freezing point--it made a huge difference in crystal lifetime.
I recall a
On Tuesday 10 July 2007 09:16, Mary Fitzgerald wrote:
If that doesn't work maybe, I'll try seeding at lower MPD
concentrations or pressure freezing.
Thanks again,
Mary
With such a high MPD condition I would probably first try micro-seeding at
25-35% MPD before doing anything else. It sounds
