Hi SnowDeer-
What's your precipitant? Have you tried lowering that as much as possible?
This will likely delay nucleation and crystal growth but the crystals will
also likely be larger (and perhaps more well ordered). Of course, you may
reach a lower limit where you prohibit nucleation. You'll
To Boaz: I seperated the large crystals and checked its diffraction already.
While the diffraction is quite poor, only several dots could be seen. T_T
I washed the seeds twice with my buffer and seed the drop immediately after
setting it. Thanks for your advices and I will try the additives.
To
SnowDeer,
Additives are indeed a good idea, make sure you do it in an informed
manner, each additive is different and you will
get a benefit only if you know how to do it right. I do not recommend a
random approach.
For example, you mention glycerol. There is a lot to know on the subject
Dear SnowDeer,
Just how small is too small? If you have access to a microfocus beamline you
might find you can collect decent diffraction data from crystals with
dimensions in the single digit microns. Fishing tiny crystals is difficult,
but something like the MicroMesh tennis racquet mounts can
Dear All:
Recently I am working on a protein which can already grow nice pyramid-like
crystals after the condition was optimized, while the crystals are too small
to be picked up. The crystals grew quite fast and densely, so I tried to put
100ul paraffin oil inside the 600ul reservoir solution or
dear SnowDeer,
The first step to see if bigger crystals can be grown is to use bigger
protein drops and vary
the precipitant/protein ratio at the beginning of the vapour diffusion
experiment.
You can work out for yourself why this
should give you all the informations that you need in
vary the precipitant/protein ratio
Agree with Enrico.
Reducing the concentration of your protein sample to make it form less
nuclei, then it might grow bigger.
Best R,
tiantian
On Mon, Sep 5, 2011 at 7:48 PM, Enrico Stura est...@cea.fr wrote:
dear SnowDeer,
The first step to see if bigger
