P4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
HanJie_HCT Tai
Sent: 17 May 2009 13:27
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] How to improve crystal which is twinning?
Hi,
I have a 22kDa protein that the floopy N & C terminus have been deleted.
It was crystallized in 35%MPD/0.
Hi Ho--
We have had lots of success using the Hampton Research Crystal Screens 1 &
2 as additives. Sometimes these improve the crystal quality whereas the
96 Additive Screen does not. Whenever I screen for additives, I include
Crystal Screen 1 & 2. What I have NOT had a great deal of succes
Hello Annie,
How effective have you found using sparse matrix screens as
additives vs. traditional additive screens? I've tried this only a few
times without success and would like to hear from someone with more
experience.
Thanks!
Ho
UC Berkeley
--
/483-3228
919/483-0368 (FAX)
annie.m.hass...@gsk.com
"HanJie_HCT Tai"
Sent by: "CCP4 bulletin board"
17-May-2009 08:27
Please respond to "HanJie_HCT Tai"
To
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Subject
[ccp4bb] How to improve crystal which is twinning?
Hi,
I have
Try things that affect nucleation and kinetics of crystallization,
such as seeding or crystallization at different temperatures. As
Morten suggested, try different crystallization methods such as
switching between hanging/sitting drop and microbatch. Screen protein
concentration and protein:crystal
what you need to
grow well ordered crystals of your protein.
- Morten
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
HanJie_HCT Tai
Sent: Sunday, May 17, 2009 8:27 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] How to improve crystal which is twinning?
Hi,
Hi,
I have a 22kDa protein that the floopy N & C terminus have been deleted. It was
crystallized in 35%MPD/0.1m Tris (pH 8.5)/0.2M/(NH4)2SO4 in 2 - 3 days.
Previously, a few small twining crystals were grown in this condition.
I tried Hampton Research 96-additive screen . Additives such
