Re: [ccp4bb] How to optimize protein-DNA complex conditions?

2010-09-13 Thread John Hinks
that's useful, John. john.hi...@syngenta.com www.syngenta.com -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Phoebe Rice Sent: 01 September 2010 23:04 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] How to optimize protein-DNA complex conditions

[ccp4bb] How to optimize protein-DNA complex conditions?

2010-09-01 Thread yang li
Dear All, Recently I am working on a protein-DNA complex, and from running agarose gel, there is a weak delayed band after the band of pure DNA which indicates some DNA has bind to the protein though the binding efficiency is low. Then I tried to optimize the condition to increase the