Dear Nishant,
are you sure your crystals are protein ? The condition contains 1 M of
different salts and PEG as well - I wouldn't exclude that you are having salt
crystals. You can easy check this with Izit dye or simply by trying to destroy
the crystal with a microtool. If it is salt you can
Niks
Seeding often works very well with needles. But start with seeding in
RANDOM SCREENS, i.e. rMMS
References -
Allan D’Arcy, Frederic Villarda, May Marsh. 'An automated microseed
matrix-screening method for protein crystallization'. Acta
Crystallographica section D63 (2007) 550–554.
4Mb attachments, posted to a mailing list NOT COOL.
On 24/07/2012 14:40, Niks wrote:
Dear All,
I am trying to crystallize a recombinant dehydrogenase protein. Got
five hits in PEG ION Screen from Hamptons (20% PEG 3350 with 0.2M
Sodium Acetate, 0.2M Potassium acetate, 0.2M ammonium
The diffraction you see is probably not the best diffraction you could
obtain from these crystals. I have found long thin needles are
very susceptible to manipulation.
I would highly recommend seeding (I like the Hampton Seed Bead personally,
24, 2012 4:02 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Improvement in crystal quality
Dear All,
I am trying to crystallize a recombinant dehydrogenase protein.
Got five hits in PEG ION Screen from Hamptons (20% PEG 3350 with 0.2M
Sodium
