Re: [ccp4bb] Linking non-natural nucleotide with other natural nucleotides

2019-05-02 Thread Eleanor Dodson
Yes - this area is a mess. However I got round it to some extent by naming the nucleotide as a standard one ((in your case C) , then adding the heavy atom, and creating the LINK records between that C and the additional atom(s). Probably at deposition the moiety should be renames as 5CM but that

[ccp4bb] Linking non-natural nucleotide with other natural nucleotides

2019-05-01 Thread Abhishek Suman
Dear experts, I am trying to insert a non-natural nucleotide (5CM, methylated cytosine) at 6th position in a 13-mer DNA. I have generated the CIF file for 5CM using ELBOW (Phenix). I tried replacing the Cytosine with 5CM and linking the O3' of -1 nucleotide with P of 5CM and o3' of 5CM with P