Subject: [ccp4bb] Low resolution structure determination advice
To: mailto:CCP4BB@JISCMAIL.AC.UK
CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK
Date: Thursday, September 1, 2011, 9:31 AM
Dear all,
We're looking for some advice about how to proceed with a structure we're
working on. Our
Dear all,
We're looking for some advice about how to proceed with a structure we're
working on. Our protein is 750 amino acids and naturally binds zinc. We have
a SeMet data set that goes down to 3.7 angstroms. 4 of 8 selenium sites are
ordered and visible in addition to our zincs and we've
Hi,
Depending on how many zn sites you have, you may be able to do zn-mad
for your native crystals. You don't mention if you've tried combining
your various sources of phase information; if not, it's worth looking into.
You may also want to look into various multi-crystal techniques
How about phase extension using DM, sure you say you only have one mol per asu
but it might still be worth trying various approaches of solvent
flattening/flipping.
Don't know what you used to detect your sites and refine them, but it also
might be worth sticking them into Sharp with your
to combine phases from
this procedure with the Selenium SAD phases, as already suggested.
Kianoush
--- On Thu, 9/1/11, Basudeb Bhattacharyya bbhattach...@wisc.edu wrote:
From: Basudeb Bhattacharyya bbhattach...@wisc.edu
Subject: [ccp4bb] Low resolution structure determination advice
To: CCP4BB
