Re: [ccp4bb] MR phasing using Negative Stain EM reconstruction

2016-10-25 Thread Stefano Trapani
Dear Pascal have a look at : https://doi.org/10.1107/S0907444910002763 Acta Cryst. (2010). D66, 514-521 Macromolecular crystal data phased by negative-stained electron-microscopy reconstructions S. Trapani, G. Schoehn, J. Navaza and C. Abergel Best, --- Stefano Trapani Maître de

Re: [ccp4bb] MR phasing using Negative Stain EM reconstruction

2016-10-25 Thread Savvas Savvides
Dear Pascal, the EM2DAM program from the ATSAS package for SAXS (https://www.embl-hamburg.de/biosaxs/manuals/em2dam.html ) can convert any EM envelope to a dummy-atom model in pdb format. Also, the recent SUPALM algorithm might provide

Re: [ccp4bb] MR phasing using Negative Stain EM reconstruction

2016-10-25 Thread Alexandre OURJOUMTSEV
traces of this are in the article in Acta Cryst D51 (1995) 888-895. Best regards, Sacha Urzhumtsev De : CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] De la part de Pascal Egea Envoyé : mardi 25 octobre 2016 03:49 À : CCP4BB@JISCMAIL.AC.UK Objet : [ccp4bb] MR phasing using Negative Stain EM

Re: [ccp4bb] MR phasing using Negative Stain EM reconstruction

2016-10-25 Thread Randy Read
Dear Pascal, I'm assuming that you're talking about using the negative stain image as an MR model. I don't recall hearing of this having ever worked (though I would be very interested of course if anyone has managed to do this!), but my intuition is that it's not going to work. Negative

[ccp4bb] MR phasing using Negative Stain EM reconstruction

2016-10-24 Thread Pascal Egea
Dear All, I would like to know if it is possible to use a low resolution EM reconstruction of a complex obtained in negative stain EM (not cryo EM) to help molecular replacement in a 4.5A resolution X-ray diffraction data set of the same complex I am aware of the possibility of using low