@JISCMAIL.AC.UK
Subject: [ccp4bb] Membrane fractionation
Dear crystallographers
When we purify membrane proteins, we often fractionate the E. coli cell
membrane by ultracentrifugation. Is there a need for this step instead of
solubilizing the whole cell with detergents?
Thank you.
Theresa
Dear crystallographers
When we purify membrane proteins, we often fractionate the E. coli cell
membrane by ultracentrifugation. Is there a need for this step instead of
solubilizing the whole cell with detergents?
Thank you.
Theresa
