Dear all
I have a His-tagged membrane protein with unknown oligomerization state. But I
am worried that tag addition may induce different state than in native and
affect its crystallizability.
Is there a single method that can determine the oligomerization state with
nearly 100% accuracy? I
Dear Theresa,
Although I haven't yet done the experiment myself, I am told that
microscale thermophoresis (MST) is another useful method for
oligomerization measurements, especially since it is designed to work for
membrane proteins in presence of detergents etc. If you want to quickly
learn more
Hello Theresa,
Maybe a functional assay (if possible) is better than determining the
oligomeric state?
Why is finding out the oligomeric state (which is unknown anyway and the
answer can be compared to nothing), the answer to is having a tag ok?
will the protein crystallize?
What if the analysis
