Hello,
Indeed, Rosetta-ddG can evaluate the effect of residue mutations. Also, have a
look at the program FoldX for per-residue evaluation of mutations
(https://foldxsuite.crg.eu/). Then, there is the freeware webtool FireProt, its
algorithm uses FoldX as a pre-filter to select beneficial
Dear all
I have a protein engineering question.
If you have a nanobody ligand complex structure is there a program that can use
the crystal structure to suggest engineering improvements to give a tighter
binding nanobody. It need not just be in the CDRs. Maybe rosetta can do this?
Any
