Re: [ccp4bb] Phaser: tNCS present but correction not applied

2016-11-26 Thread Eleanor Dodson
That is a very high nTCS vector (78% of the origin. Are you sure your unit cell isny doubled ie cell is not 94.2073 148.003 72.9967 90 97.6686 90 But 47.136 148 7390 98 90 What does pointless suggest for theSG Eleanor On 26 November 2016 at 18:56, KAUSHIK H.S. < 02b42c18251e-dmarc-requ

Re: [ccp4bb] Phaser: tNCS present but correction not applied

2016-11-26 Thread KAUSHIK H.S.
Dear Dr. Read, For the first time, Phaser gave the following warning.XXXWarning: Input/Default tNCS NMOL (2) does not match suggested NMOL (4): Please check cell content analysis and tNCS to confirm NMOL Warning: tNCS is present but correction factors NOT applied XXX Hence, I set NMOL=4 (

Re: [ccp4bb] Phaser: tNCS present but correction not applied

2016-11-26 Thread Randy Read
Hi, That message should mean that you didn't ask for a number of molecules divisible by the order of the tNCS. With that vector you need to search for an even number of copies. Let me know if that doesn't explain what you're saying. Best wishes, Randy Read Randy J. Read > On 25 Nov 20

[ccp4bb] Phaser: tNCS present but correction not applied

2016-11-25 Thread KAUSHIK H.S.
Hello, I have a crystal in C2 spacegroup (94.2073 148.003 72.9967 90 97.6686 90) and Xtriage predicts 923 residues in ASU.  My protein could be anywhere between 95 to 110 residues long (assuming the terminals could be cleaved).  Using a homolog, Phaser gives me a solution with LLG=1072 and TFZ=4