of our computer systems and checking Compliance with our Code of
Conduct and Policies.
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk]on Behalf Of Ed
Pozharski
Sent: 14 September 2009 17:47
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Preparation of seed-stocks
You can always make your own 'seed-bead' using a French Press nylon ball
(1/8) and an eppendorf.
Or in the absence of French Press consumables suppliers, just find your
local industrial gadget supplier (I found Dejay Distribution Ltd in the
UK via google). They quoted me £4.45 for 100 x 1/8 nylon
You can use the glass cell disruption pestle
http://www.vwrsp.com/catjpg/mp0/mp0440.jpg
to grind them up. They cost money, of course, and if you plan to buy
something, why not go for seed bead kit? But if you want something very
cheap, then you'll find this amazing discovery interesting:
Dear CCP4bbers,
Can anyone suggests how to make seed-stocks if one is not having
seed-beads... Is there any other methods to crush the crystals for the same
purpose. What if it is simple vortexed. Off-course there wold be all sorts
of sizes, the intact crystals as well.
Please suggest.
Thanks a
sonication
Dear CCP4bbers,
Can anyone suggests how to make seed-stocks if one is not having
seed-beads... Is there any other methods to crush the crystals for the
same
purpose. What if it is simple vortexed. Off-course there wold be all sorts
of sizes, the intact crystals as well.
Please
We used to use a needle to crush the crystals - and then make serial
dilutions
ucbccka wrote:
sonication
Dear CCP4bbers,
Can anyone suggests how to make seed-stocks if one is not having
seed-beads... Is there any other methods to crush the crystals for the
same
purpose. What if it
You can melt the end of a thin glass capillary, forming a glass 'bead' at one
end.
You can then attack your drop, containing the crystals to made into seeds, with
the beaded end of the capillary.
You can then pipette up the crushed crystals, and dilute and/or spin to make
your seed stock.
With
Hi
Most probably you might have micro seeds stock in your present well.
A streak with cat whisker/something like that in your old well and
immedietly a streak in your new well might work miracles sometimes.
HTH
all the best
Natesh.
Dear CCP4bbers,
Can anyone suggests how to make
, September 12, 2009 10:03 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Preparation of seed-stocks without seed-beads
Hi
Most probably you might have micro seeds stock in your present well.
A streak with cat whisker/something like that in your old well and immedietly a
streak in your new well might
