Dear All,
I apologize if the questions has already been asked on this forum.
We are purifying a membrane that seems prone to proteolysis. Although we use
Protease Inhibitor cocktails during lysis and the first step of purification
we get rid of them after and only keep PMSF and EDTA as general
Regarding adding inhibitors. I'm not sure the effect on crystallization but if
you use too much you will get covalent modification of your protein from one of
them (I can check my notes as to which one). We searched many MS databases of
protein modifications to realize why our protein was the
