Hi All,
I have a simple question about the complex formation between macromolecular
complex and antibody. My protein is stable in the presence of the 5mM DTT
and under these conditions the reducing environment is too strong for the
antibody to survive. I am also now trying to check the
Jan,
Are you dealing with a whole IgG or an Fab?
Most Fab-antigen complexes will not be affected by 5mM DTT.
Fab production makes use of reducing agents that allow the
antibody hinge region to become exposed to proteases.
If you need DTT to prevent your antigen from aggregating, try
to
Hi Jan,
you might mutate the Cys residues which are oxidation sensitive,
you could block the Cys thiols in your oxidation sensitive protein,
or you try crystallization at slightly acidic conditions where the Cys
thiol should be more stable (might be bad for the ab-protein complex),
or try
