: CCP4BB@JISCMAIL.AC.UK
Objet : Re: [ccp4bb] Protein precipitating at higher concentration!!
Hi Ivan
this will not be an answer to your question, but did you consider not
concentrating 'too much' your sample? It happened few times to me that the
protein was precipitating when concentrati
Hi Ivan
this will not be an answer to your question, but did you consider not
concentrating 'too much' your sample? It happened few times to me that the
protein was precipitating when concentrating for SEC because of the presence of
other impurities. Trying the good old AS precipitation helped
Hi Xtallographers,
I have been able to purify a protein that was initially going into
inclusion bodies from the excellent suggestions that I got here.
So my lysis buffer has 0.5M Guanidium Hydrochoride, 2% TritonX-100, 500mM
NaCl, 5% Glycerol in 20 mM Tris-HCL pH 8.0
The problem is that the prote
