Re: [ccp4bb] Pseudo translational symmetry Problem or Wrong Spacegroup

2012-12-07 Thread Randy Read
Dear Yurong, I just wanted to check that you're using an up-to-date version of Phaser, which will account for the presence of translational NCS (tNCS). With older versions of Phaser, you could get apparent solutions that were incorrect but would give a high LLG just because they satisfied the

Re: [ccp4bb] Pseudo translational symmetry Problem or Wrong Spacegroup

2012-12-07 Thread Edward A. Berry
Does this mean that, when placing the first (or only) copy of a model, the score will not be elevated by presence of tNCS (Unless the model contains domains separated by more or less exactly the distance of the tNCS vector)? (In general, using a generic MR program that does not correct for TNS.)

[ccp4bb] Pseudo translational symmetry Problem or Wrong Spacegroup

2012-12-03 Thread Yurong Wen
Dear All, Recently, I collected a dataset of a protein-DNA complex and indexed in spacegroup P212121 to 3.4 Å. However, Phenix.Xtriage indicated the presence of a very high pseudotranslational symmetry peak. I scaled the data in spacegroup P222. When I used the protein heterotetramer as

Re: [ccp4bb] Pseudo translational symmetry Problem or Wrong Spacegroup

2012-12-03 Thread Herman . Schreuder
@JISCMAIL.AC.UK Subject: [ccp4bb] Pseudo translational symmetry Problem or Wrong Spacegroup Dear All, Recently, I collected a dataset of a protein-DNA complex and indexed in spacegroup P212121 to 3.4 Å. However, Phenix.Xtriage indicated the presence