padu 2017 12:01:43
Komu: CCP4BB@JISCMAIL.AC.UK
Předmět: [ccp4bb] Radiation damage to the FAD in enzyme structure
Dear colleagues,
I am investigating a structure of a FAD-dependent enzyme. The electron density
map suggests radiation damage to the FAD. It apparently is different from
s
<malym...@fjfi.cvut.cz>
Reply-To: Martin Malý <malym...@fjfi.cvut.cz>
Date: Tuesday, 7November, 2017 at 05:16
To: "CCP4BB@JISCMAIL.AC.UK" <CCP4BB@JISCMAIL.AC.UK>
Subject: Re: [ccp4bb] Radiation damage to the FAD in enzyme structure
Dear Ilme,
thank you for your answer. We ar
,
Martin Maly
Od: Ilme Schlichting <ilme.schlicht...@mpimf-heidelberg.mpg.de>
Odesláno: 6. listopadu 2017 12:38:17
Komu: Martin Malý
Předmět: Re: [ccp4bb] Radiation damage to the FAD in enzyme structure
Hello,
Are you sure you had full occupancy to begin wit
Dear Martin,
A more bleeding-edge type of experiment in the vein David is
indicating would be to use a "rotation SFX" protocol to record
complete data off one or very few crystals that will be completely
exempt from radiation damage, as described in doi:10.1038/nmeth.2962
and in doi:
Some alternative interpretations have been suggested, but if you think
you are seeing radiation damage, you could try collecting data on
several crystals and binning it by dose received. For comparison see:
The catalytic pathway of horseradish peroxidase at high resolution.
Berglund, et al.
..@fjfi.cvut.cz>
Date: Monday, 6November, 2017 at 03:01
To: "CCP4BB@JISCMAIL.AC.UK" <CCP4BB@JISCMAIL.AC.UK>
Subject: [ccp4bb] Radiation damage to the FAD in enzyme structure
Dear colleagues,
I am investigating a structure of a FAD-dependent enzyme. The electron density
map su
Dear Martin,
we experienced the butterfly-like structure of FAD in monoamine oxidases
but this was observed also in other flavoenzymes. Whether this is an
intrinsic feature of these enzymes or something due to radation damage is
not clear for each case. However, in our experience this bent
Dear Martin,
a couple of years ago we had a similar problem with FAD degradation. In
our case it was not caused by radiation damage, but we saw different
ratios of intact and degraded FAD depending on the age of the crystals
[Winkler et al., Nat. Chem. Biol. 4, 739-741 (2008)].
Best
Dear colleagues,
I am investigating a structure of a FAD-dependent enzyme. The electron density
map suggests radiation damage to the FAD. It apparently is different from
simple change of the redox state and "butterfly"-like structure. We did not
find in literature possible products of
