Re: [ccp4bb] Reducer and crystallization

Hi Sam, The effect of reducing agents like beta-ME, DTT or TCEP is exactly as you say, the prevention of disulphide formation or cleavage of existing disulphide bridges and therefore prevention of aggregation if inter-molecular disulphide bridges can be formed. A balancing of reducing power, or

[ccp4bb] Reducer and crystallization

Dear all, Can anyone enlighten me the effect of reducer in crystallography? I understand that it removes disulphide bond, and prevent protein aggregation. But how do we find a balance point and must we remove it before screening crystals? Thanks for answering first. Cheers sam