gt;
> From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of aka
> akaka
> Sent: Monday, February 09, 2009 7:27 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] Se oxidation
>
>
>
> Dear All
>
>
>
> I would like to know whethe
Dear all,
one comment on the subject of white lines and anomalous phasing
power
- one has to be extremely careful to jump to conclusions
about the effect that a chemical treatment of the heavy atom (say
oxydation/reduction of Se) has
on the shape of the edge and the associated anomalous signa
bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of aka
akaka
Sent: Monday, February 09, 2009 7:27 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Se oxidation
Dear All
I would like to know whether oxidation of Se entails any problem for SAD or
MAD experiments and/ or how to resolve it
Hi,I believe it is not important as long as you run a proper scan of the
crystal. Both forms will allow proper phasing.
This is very well described in a paper from Thomazeau et al.
here is the reference
MAD on threonine synthase: the phasing power of oxidized selenomethionine.
Acta Crystallogr D B
Hi,
I wouldn't bother either. I once phased a structure based on about 160
oxidized Se in the AU (Hothorn et al., JBC, 2006). Just make sure that
most of them are oxidized and do a proper absorption scan.
best
Michael
aka akaka wrote:
Dear All
I would like to know whether oxidation of Se
2009 1:27 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Se oxidation
Dear All
I would like to know whether oxidation of Se entails any problem for SAD or MAD
experiments and/ or how to resolve it. Cannot use DTT or reducing agents in my
protein (extracellular and disulphide bonds are importa
Dear AllI would like to know whether oxidation of Se entails any problem for
SAD or MAD experiments and/ or how to resolve it. Cannot use DTT or reducing
agents in my protein (extracellular and disulphide bonds are
important).ThanksDr. R.DepetrisWeill Cornell Medical College
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