Hi Kristof
We had an example a few years back, with a Co ion leakage off an old Talon
resin necessary for the crystal to grow (until we realized you could just
add Co instead to get crystals to grow).
The ion was used in the end as anomalous scatterer to solve the structure,
and was found bound
Dear,
I was wondering if anybody has experienced before the leakage of Ni-
ions (from a Ni-NTA column) and additionally binding to specific
sites in the protein structure..?
Many thanks
Regards
Kristof
--
Kristof Van Hecke, PhD
Biomoleculaire
Hi Kristof,
depending on what you termed specific sites the answer is definitely
yes.
I experienced such a case in the following reference related to the
AcrB structure but I remember that this situation arose also in the
case of the LTC4 structure.
-There is a baby in the bath water:
