Glycerol is known to be able to reduce nucleation. This might be countered
by an increase in protein concentration.
Vera, L., Czarny, B., Georgiadis, D., Dive, V., Stura, E.A. (2011)
Practical Use of Glycerol in Protein Crystallization. Cryst. Growth Des.
11: 2755–2762.
Enrico.
On Tue, 03
Florian Schmitzberger wrote:
Dear Toby,
I don't think there is a basic problem using glycerol in
crystallization. Glycerol will affect the vapour pressure (if it is not
present in the well/precipitant solution) and 10 % glycerol is ~ 1.3
molar concentration. During equilibration the drops may
Dear all,
My question is related to a sample preparation.
I’m working with a complex that can be stabilized with glycerol (at least
10%) during purification. The use of detergents does not help. After
purification, the sample is homogeneous (EM) and can be concentrated
(3-4mg.mL-1) . I already
Dear Toby,
I don't think there is a basic problem using glycerol in
crystallization. Glycerol will affect the vapour pressure (if it is
not present in the well/precipitant solution) and 10 % glycerol is ~
1.3 molar concentration. During equilibration the drops may increase
in volume,
: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Florian
Schmitzberger
Sent: Tuesday, April 03, 2012 11:07 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Substitution to glycerol during crystallogenesis
Dear Toby,
I don't think there is a basic problem using glycerol
