ahw78 mailto:armin.wag...@diamond.ac.uk>>
> Date: 2 August 2018 at 09:17:48 BST
> To: Eleanor Dodson
> mailto:eleanor.dod...@york.ac.uk>>
> Cc: ccp4bb mailto:ccp4bb@jiscmail.ac.uk>>
> Subject: Re: [ccp4bb] Sulphate or phosphate?
> Dear Eleanor,
>
> We can tune
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Begin forwarded message (on behalf of Armin Wagner)
From: ahw78 mailto:armin.wag...@diamond.ac.uk>>
Date: 2 August 2018 at 09:17:48 BST
To: Eleanor Dodson mailto:eleanor.dod...@york.ac.uk>>
Cc: ccp4bb mailto:ccp4bb@jiscmail.ac.uk>>
Subject: Re: [ccp4bb] Sulphate or phosphate?
Dea
it
better.
Best regards,
Armin
[cid:image001.png@01D42A41.AE0B8B60]
From: Eleanor Dodson
Date: Wednesday, 1 August 2018 at 19:09
To: Armin Wagner
Cc: "ccp4bb@jiscmail.ac.uk"
Subject: Re: [ccp4bb] Sulphate or phosphate?
bridge which should illustrate it
better.
Best regards,
Armin
[cid:image001.png@01D42A41.AE0B8B60]
From: Eleanor Dodson
Date: Wednesday, 1 August 2018 at 19:09
To: Armin Wagner
Cc: "ccp4bb@jiscmail.ac.uk"
Subject: Re: [ccp4bb] Sulphate or phosphate?
I am not sure if you
I am not sure if you gave your wave length but it is always worth doing an
anomalous map, and looking at relativr peak heights for your known S
positions and the putative sulphate or phosphate.
There are small differences in the expected f" at most wavelengths.
Chemical arguments are doubtless bet
Dear David,
The long-wavelength beamline I23 at Diamond
(http://www.diamond.ac.uk/Instruments/Mx/I23.html) can go all way down to the
phosphorous edge. While data quality will be obviously compromised by
absorption effects (we are working on this), for a reasonably well diffracting
crystal lik
not a
mixture of both.
Regards,
Vaheh Oganesyan, PhD
www.medimmune.com
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of David
Schuller
Sent: Tuesday, July 31, 2018 9:39 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Sulphate or phosphate?
How
Sent: Tuesday, July 31, 2018 3:38:38 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Sulphate or phosphate?
How can one distinguish between a sulphate or phosphate in an electron
density map? Both are present in the mother liquor, and resolution is in
the range of 1.75 - 2.25 A
You might be able to distinguish sulfate from phosphate by examining
hydrogen bonding partners. Phosphate can donate one or two hydrogen bonds
at neutral pH values, whereas sulfate is usually only a hydrogen bond
acceptor. (Having said that, we have published a structure where a sulfate
clearly int
How can one distinguish between a sulphate or phosphate in an electron
density map? Both are present in the mother liquor, and resolution is in
the range of 1.75 - 2.25 A
--
===
All Things Serve the Beam
==
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