Hard to say without more information.
Have you refined the B factors for these residues? Most building
software gives some arbitrary b value, which must then be refined. (In
fact after any rebuilding activity you need to do a few cycles of
refinement before looking at the maps again)
Hi James,
I did check teh B-factors and they are similar to the flanking regions (about
40). The difference density appeared at the later stage of refinement (TLS and
restrained in CCP4i).
What do you think and how to do it?
Best,
Sun Tang
James Irving [EMAIL PROTECTED] wrote: Hi Sun,
I
Hello Everyone,
When I refined a structure, I found strong difference density Fo-Fc at 3
sigma contour for the for five residues which already have occupancy of 1. The
density is continuous and so strong as if I did not put the residues there. Why
was that? Can I put greater than 1
