Hi Maria,
As mentioned by Tony, it could be a chaperonin. Having little of ATP (0.5mM
or less) and Mg2+ (1mM) in lysis buffer might help.
Good Luck,
Partha
2012/3/22 SANCHEZ BARRENA, MARIA JOSE
> Dear all,
>
> I am trying to express a eukatiotic protein (E. coli codon optimized
> sequence) wit
P4BB@JISCMAIL.AC.UK>>
Subject: [ccp4bb] contaminant when overexpressing a GST tagged protein
Dear all,
I am trying to express a eukatiotic protein (E. coli codon optimized sequence)
with a GST tag at the N-terminus. I always get my overexpressed protein and a
contaminant around 60kDa. This
Dear all,
I am trying to express a eukatiotic protein (E. coli codon optimized sequence)
with a GST tag at the N-terminus. I always get my overexpressed protein and a
contaminant around 60kDa. This contaminant is not washed out of the column when
washing glutathione beads with 1M NaCl-buffer.
