An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] crystallization of complex and ...
Dear all,
I have two questions:
First, I was trying to crystallize a complex of two proteins. Both proteins
has been crystallized before. The two proteins bind to each other based on
Biacore study
Dear Min,
Regarding the stoichiometry that you should use in crystallizing two
proteins
that form a complex. I have looked at this question before. See:
Stura, E.A., Graille, M., Taussig, M.J., Sutton, B.J. Gore, M.G.,
Silverman, G.J., Charbonnier, J.-B. (2001)
Crystallization of
On Thu, 2011-09-15 at 22:20 -0400, m zhang wrote:
Second is about reusing of Ni-NTA resin. According to Qiagen's
instruction, after using fresh Ni-NTA resin, one only needs to wash
the used Ni resin first with 0.5M NaOH, then with your own buffer.
After that the resin is ready to be reused
1 imidazole affinity is not high which is why you use 200 mM or more to
elute. So it comes off by itself.
2 you can wash with low ph and then recharge this is somewhat easier on the
resin
On Sep 15, 2011 9:25 PM, m zhang mzhang...@hotmail.com wrote:
Dear all,
I have two questions:
First, I
with
to co-crystallize?
-Yes, I use the same buffer system for both proteins and complex.
Thank you all for your suggestions.
Min
Date: Fri, 16 Sep 2011 09:13:46 +0200
From: alexander.paut...@boehringer-ingelheim.com
Subject: [ccp4bb] AW: [ccp4bb] crystallization of complex and ...
To: CCP4BB
Dear all,
I have two questions:
First, I was trying to crystallize a complex of two proteins. Both proteins has
been crystallized before. The two proteins bind to each other based on Biacore
study, but they didn't form a single peak on gel filtration. When I mixed them
at 1:1 ratio, the
