[ccp4bb] crystallization of highly-glycosylated protein complex-avoid phase separation

2010-03-03 Thread Jerry McCully
Dear ALL: Recently we've been trying hard to crystallize a highly glycosylated protein complex ( 30% percent of carbohydrate in the total 120KD molecular weight). IT is a high affinity protein complex. One component can be crystallized in high salt condition and the other can be

Re: [ccp4bb] crystallization of highly-glycosylated protein complex-avoid phase separation

2010-03-03 Thread Prince, D Bryan
To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] crystallization of highly-glycosylated protein complex-avoid phase separation Dear ALL: Recently we've been trying hard to crystallize a highly glycosylated protein complex ( 30% percent of carbohydrate in the total 120KD molecular weight