Rashmi,
To facilitate dissolution of a peptide we found ultrasound (in glass, plastics
absorb much of the energy) useful. It might be also helpful to warm the
solution mildly.
Frank
Hello everybody,
I have a 16 mer peptide which is predicted to be positively charged alpha
helix and has 50% of its sequence hydrophobic.
Could any one recommend the best way to dissolve it, and then it can be used
for crystallization trials.
thanks
--
rashmi
Rashmi,
You can try 1%DMSO and also acetonitrile but the former is preferable.
Gauri
On Mon, Jun 7, 2010 at 3:04 AM, rashmi panigrahi
rashmi.panigrah...@gmail.com wrote:
Hello everybody,
I have a 16 mer peptide which is predicted to be positively charged alpha
helix and has 50% of its