Re: [ccp4bb] dissolving peptides !

2010-06-09 Thread Frank Niesen
Rashmi, To facilitate dissolution of a peptide we found ultrasound (in glass, plastics absorb much of the energy) useful. It might be also helpful to warm the solution mildly. Frank

[ccp4bb] dissolving peptides !

2010-06-07 Thread rashmi panigrahi
Hello everybody, I have a 16 mer peptide which is predicted to be positively charged alpha helix and has 50% of its sequence hydrophobic. Could any one recommend the best way to dissolve it, and then it can be used for crystallization trials. thanks -- rashmi

Re: [ccp4bb] dissolving peptides !

2010-06-07 Thread gauri misra
Rashmi, You can try 1%DMSO and also acetonitrile but the former is preferable. Gauri On Mon, Jun 7, 2010 at 3:04 AM, rashmi panigrahi rashmi.panigrah...@gmail.com wrote: Hello everybody, I have a 16 mer peptide which is predicted to be positively charged alpha helix and has 50% of its