Thanks a lot for your suggestions.
Would it be worth while to try and remove glycerol at the last step which is
gel filtration and then set trays in my case?
I have never tried without glycerol.
reg.
Anita
On Fri, Sep 9, 2011 at 7:54 PM, Enrico Stura est...@cea.fr wrote:
Anita,
see the
On Sat, 2011-09-10 at 15:00 +0800, anita p wrote:
Would it be worth while to try and remove glycerol at the last step
which is gel filtration and then set trays in my case?
Sure, assuming that your protein does not become unstable without
glycerol (protein solubility is likely to decrease
Dear Crystallographers,
I have set hanging drop trays with 2ul of protein and 2 ul of resorvior
solution. I have seen in some cases the drops are swelling. My protein
buffer has 15% glycerol in it.
This is happening mainly when I have peg 400 or peg MME or MPD or Jeffamine
in the buffer condition.
Yes - add glycerol to the reservoir -
15% glycerol has a huge influence on the vapour diffusion gradients
Poul
On 09/09/2011, at 13.22, anita p crystals...@gmail.com wrote:
Dear Crystallographers,
I have set hanging drop trays with 2ul of protein and 2 ul of resorvior
solution. I have
The following is simply a quote from a recent post by Enrico Stura.
Probably has a lot to do with what you are finding:
Glycerol is also great to reduce nucleation. If you decide to add
glycerol to the protein solution (for solubility, but in your case it
might be for stability
reasons), you
BlackBerry by ATT
-Original Message-
From: anita p crystals...@gmail.com
Sender: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK
Date: Fri, 9 Sep 2011 19:22:53
To: CCP4BB@JISCMAIL.AC.UK
Reply-To: anita p crystals...@gmail.com
Subject: [ccp4bb] drops swelling
Dear Crystallographers,
I have set
Anita,
see the message I posted yesterday on the CCP4bb:
http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg22638.html
or google stura glycerol
Re: [ccp4bb] How to help crystal grow bigger
In your case the sentence you need is:
You need to have a higher (double) glycerol concentration in the
