Hi Len
In every case that I know of this problem has been solved by working under
oil. The oil becomes saturated with the volatiles, and it prevents
crystals from doing backstroke, breaststroke or front crawl during
harvesting.
The most convenient way to get going is to dispense the wells
This paper might be helpful.
1. J Struct Biol. 2014 Nov;188(2):102-6. doi: 10.1016/j.jsb.2014.09.011. Epub
2014
Oct 5.
Efficient cryoprotection of macromolecular crystals using vapor diffusion of
volatile alcohols.
Farley C, Juers DH.
Author information:
(1)Department of Physics, Whitman
Hi All,
We have gotten some very nicely formed crystals out of a couple of different
volatile solvents recently. Besides looking for something easier to work in
does anybody have any tips on handling crystals from these types of solvents.
It is very hard to loop a crystal while it is doing
