Re: [ccp4bb] how to optimize crystal without sharp boundary

2009-05-20 Thread Kay Diederichs
Hi Jerry, did you check out the CCP4 wiki - there's a lot of information at http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Crystals HTH, Kay Jerry McCully schrieb: Dear All: Recently we got some crystals from ammonium sulfate and PEG400. They look like hexagonal single

[ccp4bb] how to optimize crystal without sharp boundary

2009-05-20 Thread Jerry McCully
Dear All: Recently we got some crystals from ammonium sulfate and PEG400. They look like hexagonal single crystals but without sharp boundary. They did not diffract well. This protein can also be crystallized from PEG6000 in star shape. We tried hampton additive screen but can