Hydroxyapatite can be miraculous, but is often very capricious: among
others, it is temperature sensitive and two different corners of a
somewhat inhomogeneous cold room may provide different results.
Daniel
Le 29/11/2010 16:08, Sebastiano Pasqualato a écrit :
Hi all,
I read/heard that hydrox
Hi Sebastiano,
Just to add to Dima's response, I've used ceramic hydroxyapatite column and
got great results separating 2 very large membrane proteins complexes that
eluted together in DEAE/Q and ran in similar size range in SEC. One eluted
in 100-200mM KPi and the the other in 300mM+ KPi ~pH 7.4.
I was wondering if any of you would be kind enough to share her/his
experience with me, and would suggest vendors and models for such columns.
I really like "ceramic" hydroxyapatite from Bio-Rad. The only type that
behaves in the columns long-term, without the need for repacking. It also
gives
Hi all,
I read/heard that hydroxyapatite column can be used to purify proteins, getting
separation results orthogonal to ion exchange and size exclusion chromatography.
I was wondering if any of you would be kind enough to share her/his experience
with me, and would suggest vendors and models for
